Technical Data
C2097-03B
Cathepsin B (Cathepsin-B, CPSB, CTSB, Cathepsin B1, Amyloid Precursor Protein Secretase, APP Secretase, APPS, Preprocathepsin B, Procathepsin B)
Description:
Cathepsin B is thought to play an increasingly important role in apoptosis and its release from the lysosome under cell stress leads to Bid cleavage, cytochrome C release and nuclear fragmentation. Cathepsin B is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete processing of APP is thought to be a causative factor in Alzheimer disease.

Overexpression of cathepsin B has been associated with oesophageal adenocarcinoma and other tumors, and has in particular been implicated in tumor invasion and metastasis.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:2,000-1:20,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml4C/-20C (No Glycerol)Blue IceHumanSheep
Concentration:
~5mg/ml
Immunogen:
Native, from placenta
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human CATHEPSIN B.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.