Technical Data
C2097-05
Cathepsin B
Description:
Cathepsin B is a lysosomal cysteine proteinase that is important for intracellular protein catabolism. Cathepsin B has been implicated in several disease states including muscular dystrophy, rheumatoid arthritis and tumor metastasis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommnended Dilutions:
Western Blot: 2-4ug/ml. Detected both procathepsin B and cathespin B in 20ug of rat kidney microsomal preparation (M3893-27). Rat kidney microsomal preparation was resolved by electrophoresis, transferred to PVDF and probed with C2097-05. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: 10ug/ml positively stained rat kidney and liver sections that had been fixed with ethanol:acetic acid [95:5] for 5 minutes at RT.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to rat pro-Cathepsin B
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes rat pro-Cathepsin B (~40kD) and mature Cathepsin B (25, 26 and 30kD). Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rowan, A.D., et al., Biol. Chem. Hoppe-Seyler 373: 427432 (1992). 2. Lee, E.R., et al., J. Histochem. Cytochem. 43: 525536 (1995).