Technical Data
C2097-05
Cathepsin B
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Technical Data |
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C2097-05 |
Cathepsin B |
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Description: Cathepsin B is a lysosomal cysteine proteinase that is important for intracellular protein catabolism. Cathepsin B has been implicated in several disease states including muscular dystrophy, rheumatoid arthritis and tumor metastasis. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommnended Dilutions: Western Blot: 2-4ug/ml. Detected both procathepsin B and cathespin B in 20ug of rat kidney microsomal preparation (M3893-27). Rat kidney microsomal preparation was resolved by electrophoresis, transferred to PVDF and probed with C2097-05. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunohistochemistry: 10ug/ml positively stained rat kidney and liver sections that had been fixed with ethanol:acetic acid [95:5] for 5 minutes at RT. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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