		Technical Data
C2097-10	Cathepsin B

Description:
Cathepsin B is a cysteine protease commonly overexpressed in metastisizing cancer cells. An intracellular (lysosomal) enzyme in peptidase family C1 (papain family), Cathepsin B will hydrolyze proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA (Indirect): 1:1024
Western Blot: 1:4000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice	Human	Rabbit

Concentration:

~4mg/ml

Immunogen:

Cathepsin B (Human liver).

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

Specificity:

Recognizes human liver Cathepsin B. Does not crossreact with Cathepsin L, Cathespin D or Cathepsin H.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.