Technical Data
C2097-11
Cathepsin B
Description:
Cathepsin B plays a role in protein degradation and has also been shown to activate urokinase type plasminogen activator (uPA) and certain matrix metalloproteinases (MMPs).

Applications:
Suitable for use in Immunohistochemistry. Not suitable for use in Western Blot or frozen sections. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:40-1:80 No pretreatment.
Optimal dilution determined by the researcher.

Hybridoma:
Spleen cells form immunized mice were fused with cells of the p3-NSI-Ag4-1 myeloma cell line.

Recommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:HRP

Recommended Positive Control Tissue:
Liver

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b6A221Supernatant
SizeStorageShippingSourceHost
1ml4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant human cathepsin B.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in PBS, 0.09% sodium azide. Reconstitute with 1ml dH2O or 40-50% glycerol..
Specificity:
Specific for Cathspsin B, a lysosomal protease expressed in a wide range of tissues, including liver, skeletal muscle and heart.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.