Technical Data
Cathepsin B (Procathepsin B)
Cathepsin B is a cysteine protease commonly overexpressed in metastisizing cancer cells. An intracellular (lysosomal) enzyme in peptidase family C1 (papain family), Cathepsin B will hydrolyze proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.

Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:200. Paraffin sections after microwave treatment.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG16D231Affinity Purified
200ug-20CBlue IceHumanMouse
Recombinant human procathepsin B.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.01% thimerosal.
Recognizes the epitope E212PGYSP217 of human cathepsin B. Recognizes mature human single or two chain cathepsin B and procathepsin B. Does not crossreact with mature cathepsins H, K, L, S, V, W or procathepsins L, S, W.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Contact of high-invasive, but not low-invasive, melanoma cells to native collagen I induces the release of mature cathepsin B: A. Klose, et al.; Int. J. Cancer 118, 2735 (2006)