Technical Data
C2097-24B
Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311)
Description:
The aspartic protease cathepsin D is one of the major proteolytic enzymes in lysosomes. The cathepsin D gene locates on chromosome 11p15.5 and spans approximately 11 kb with nine exons. Initially synthesized as an inactive precursor of pro-cathepsin D, the enzyme is subsequently converted to its active forms by proteolytic processing. Breast cancer cells, unlike normal cells, secrete high levels of pro-cathepsin D. Cathepsin D has been reported to be involved in malignant tumor progression and with prognosis of various human cancers.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000
Immunohistochemistry: 1:50-1:100.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control Tissue:
Melanoma, invasive ductal carcinoma

Hybridoma:
Derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human cathepsin D protein.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k6j3Affinity Purified
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant human Cathepsin D (21-412 aa) purified from E. coli
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human Cathepsin D at 45kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Tang,J. Mol. Cell. Biochem. 26: 93-109 (1979). 2.Fujita, H et al., Biochem. Biophys. Res. Commun. 179: 190-196 (1991). 3.Fusek, M et al., Biomed Papers 149: 43-50 (2005). 4.Garcia,M et al., Stem Cells 14: 642-650 (1996). 5. Rochefort, H et al., Cancer Cells 2: 383-388 (1990).