		Technical Data
C2097-24B	Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311)

Description:
The aspartic protease cathepsin D is one of the major proteolytic enzymes in lysosomes. The cathepsin D gene locates on chromosome 11p15.5 and spans approximately 11 kb with nine exons. Initially synthesized as an inactive precursor of pro-cathepsin D, the enzyme is subsequently converted to its active forms by proteolytic processing. Breast cancer cells, unlike normal cells, secrete high levels of pro-cathepsin D. Cathepsin D has been reported to be involved in malignant tumor progression and with prognosis of various human cancers.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000
Immunohistochemistry: 1:50-1:100.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control Tissue:
Melanoma, invasive ductal carcinoma

Hybridoma:
Derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human cathepsin D protein.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b,k	6j3	Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Recombinant human Cathepsin D (21-412 aa) purified from E. coli

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.

Specificity:

Recognizes human Cathepsin D at 45kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Tang,J. Mol. Cell. Biochem. 26: 93-109 (1979). 2.Fujita, H et al., Biochem. Biophys. Res. Commun. 179: 190-196 (1991). 3.Fusek, M et al., Biomed Papers 149: 43-50 (2005). 4.Garcia,M et al., Stem Cells 14: 642-650 (1996). 5. Rochefort, H et al., Cancer Cells 2: 383-388 (1990).