Technical Data
Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311 )
Cathepsin D is an aspartyl protease expressed in all cells and has a role on the degradation of proteins in lysosomes. It is initially expressed as an inactive 52kD precursor and activated by proteolysis.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Band at 34kD and weaker band at 52kD
Immunohistochemistry (Frozen sections and paraffin-embedded sections): 1:200 for paraffin sections
Optimal dilutions to be determined by the researcher.

Recommended Positive Controls:
Human breast carcinoma or cell line extract.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C (-20C Glycerol)Blue IceHumanMouse
Not Determined
Full length purified native Cathepsin D from human liver. Cellular Localization: Lysosomal.
Supplied as a liquid, 15mM sodium azide
Recognizes human Cathepsin D. Does not react with bovine cathepsin D and B, or with human cathepsin types B, C, G or H.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Igdoura SA et al. Differential expression of cathepsins B and D in testis and epididymis of adult rats. J Histochem Cytochem 43:545-57 (1995). 2. Westley B & Rochefort H A secreted glycoprotein induced by estrogen in human breast cancer cell lines. Cell 20:353-62 (1980).