Technical Data
C2097-24F
Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311 )
Description:
Cathepsin D is an aspartyl protease expressed in all cells and has a role on the degradation of proteins in lysosomes. It is initially expressed as an inactive 52kD precursor and activated by proteolysis.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Band at 34kD and weaker band at 52kD
Immunohistochemistry (Frozen sections and paraffin-embedded sections): 1:200 for paraffin sections
Optimal dilutions to be determined by the researcher.

Recommended Positive Controls:
Human breast carcinoma or cell line extract.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a8J539Ascites
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Full length purified native Cathepsin D from human liver. Cellular Localization: Lysosomal.
Purity:
Ascites
Form
Supplied as a liquid, 15mM sodium azide
Specificity:
Recognizes human Cathepsin D. Does not react with bovine cathepsin D and B, or with human cathepsin types B, C, G or H.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Igdoura SA et al. Differential expression of cathepsins B and D in testis and epididymis of adult rats. J Histochem Cytochem 43:545-57 (1995). 2. Westley B & Rochefort H A secreted glycoprotein induced by estrogen in human breast cancer cell lines. Cell 20:353-62 (1980).