Technical Data
C2097-91
Caveolin 1 (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)
Description:
Caveolin describes a group of 22-24kD integral membrane proteins that are localized to caveolae on the plasma membrane. Caveolae are specialized flask-shaped invaginations of the plasma membrane that are thought to exist in all cell types, but are most prominent in adipocytes, endothelial cells, muscle cells and fibroblasts. Caveolae are thought to play a role in cell signaling and in transcytosis across the plasma membrane. There are three groups of caveolin proteins (caveolin-1, -2 and -3). The gene for caveolin-1 encodes both -1 and -2. Caveolin-3 is expressed primarily in muscle cells. Although caveolin localizes to the plasma membrane, it is insoluble in non-ionic detergents and is believed to be a structural component involved in caveolae assembly. In addition to its structural role, caveolin binds to cholesterol and to G-alpha subunits of heterotrimeric G-proteins. Originally identified with anti-phosphotyrosine antibodies, caveolin is phosphorylated on tyrosine by the Src tyrosine kinase. Phosphorylated caveolin can be detected in v-Src transformed cells, and in cells stimulated with insulin. However, the effect of tyrosine phosphorylation on caveolin function remains to be determined.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 2-4ug/ml
Western Blot: 0.5-2ug/ml
Immunoprecipitation: 2-5ug/IP
Optimal dilutions to be determined by the researcher.

Positive control: Human broblasts cell lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13G69Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Phosphorylated proteins from chick embryo broblast.
Purity:
Highly purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human caveolin 1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Denker SP, et al. J Cell Biol 133(5):10271040, 1996. 2. Luetterforst R, et al. J Cell Biol 145:14431459, 1999.