Technical Data
C2097-91A
Caveolin 1 (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 2-8ug/ml Detects caveolin-1 and isoforms in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2097-71A (2ug/ml). Proteins were visualized using aIgG (HRP) GtxMo and a chemiluminescence detection system.
Immunoprecipitation: Immunoprecipitates caveolin-1 from rat adipocyte plasma membrane (1).
Immunocytochemistry: Detects caveolin-1 in 3T3-L1 adipocyte cells (1).
Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
A0001-50: Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

*Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b4H312Affinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceRatMouse
Concentration:
~0.25mg/ml
Immunogen:
Intracellular membrane protein containing vesicles (containing GLUT4) from rat adipocytes.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes c rat aveolin-1, Mr ~24kD (caveolin-1a) and 21kD (caveolin-1b) isoforms. A non-specific protein was also detected, Mr ~105kD. Species Crossreactivity: Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Souto, R.P., et al., J. Biol. Chem. 278: 18321-18329, (2003). 2. Liu, P., et al., J. Biol. Chem. 277: 41295-41298, (2002).