Technical Data
C2097-91C
Caveolin 1 (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)
Description:
Caveolins make up a family of proteins that are principal structural components of hairpin-like domains in the plasma membrane (1). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Three members of caveolins (caveolin-1, -2 and -3) have been identified, possessing different tissue distributions (2). Caveolins interact with multiple signaling molecules, such as the G-protein alpha subunit (1), tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (2,3). Caveolin-1 has been implicated in the pathogenesis of mammary epithelial cell hyperplasia (4).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:20,000
Immunohistochemistry: 1:250. Antigen retrieval required.
Immunocytochemistry: 1:100

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k85Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues near C-terminus of human Caveolin-1
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, containing 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human Caveolin-1. Should recognize both alpha and beta forms of Caveolin 1. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Glenney, Jr., J.R. FEBS Lett. 314: 45–8 (1992).2. Okamoto, T., et al. C J. Biol. Chem. 273: 5419–5422 (1998).3. Smart, E.J., et al. Mol. Cell. Biol. 19: 7289–7304 (1999).4. Lee, H., et al. Am J Pathol. 161: 1357–69 (2002).