		Technical Data
C2097-91C	Caveolin 1 (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)

Description:
Caveolins make up a family of proteins that are principal structural components of hairpin-like domains in the plasma membrane (1). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Three members of caveolins (caveolin-1, -2 and -3) have been identified, possessing different tissue distributions (2). Caveolins interact with multiple signaling molecules, such as the G-protein alpha subunit (1), tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (2,3). Caveolin-1 has been implicated in the pathogenesis of mammary epithelial cell hyperplasia (4).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:20,000
Immunohistochemistry: 1:250. Antigen retrieval required.
Immunocytochemistry: 1:100

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	6k85	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

A synthetic peptide corresponding to residues near C-terminus of human Caveolin-1

Purity:

Supernatant

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, containing 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Specificity:

Recognizes human Caveolin-1. Should recognize both alpha and beta forms of Caveolin 1. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Glenney, Jr., J.R. FEBS Lett. 314: 45–8 (1992).2. Okamoto, T., et al. C J. Biol. Chem. 273: 5419–5422 (1998).3. Smart, E.J., et al. Mol. Cell. Biol. 19: 7289–7304 (1999).4. Lee, H., et al. Am J Pathol. 161: 1357–69 (2002).