Technical Data
C2097-98A
Caveolin 3 (CAV 3, CAV3, Caveolin3, LGMD1C, M caveolin, MGC126100, MGC126101, MGC126129, VIP 21, VIP21)
Description:
This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin-embedded sections): 1:500
Western Blot: 1ug/ml. Detects caveolin 3 from rat heart homogenate at ~21kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-18, M(1)MTEEHTDLEARIIKDIH(18) C, of human caveolin-3. This sequence is completely conserved in mouse caveolin-3.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 1mg/ml BSA., 0.05% sodium azide
Specificity:
Recognizes human caveolin-3. Does not react with caveolin-1 or -2. Species Crossreactivity: rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tang Z et al. Molecular cloning of caveolin-3, a novel member of the caveolin gene family expressed predominantly in muscle. J Biol Chem 271:2255-61 (1996).