Technical Data
C2098-85C
CBX5 (Chromobox Protein Homolog 5, Antigen p25, HP1 alpha, HP1A, Heterochromatin Protein 1 Homolog alpha)
Description:
CBX5, also known as Chromobox protein homolog 5, is a regulatory protein belonging to the Polycomb group proteins family with two chromo domains. CBX5 associates with chromosomes during mitosis, specifically with chromatin during metaphase and anaphase. A component of the centromeric and pericentromeric heterochromatin, CBX5 may interact with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. CBX5 recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. This methyl-lysine binding protein localized at heterochromatin sites is also known to mediate gene silencing and is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HCT-116

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa150-200 of human CBX5.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human CBX5. Species Crossreactivity: canine, monkey, mouse. Species sequence homology: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bannister, AJ. et al. Nature. 410:120-124 (2001). 2. Hall, IM. et al. Science. 297:2232-2237 (2002). 3. Smallwood, A. et al. Genes Dev. 21:1169-1178 (2007).