Technical Data
C2098-85E
CBX5 (Chromobox Protein Homolog 5, Antigen p25, HP1 alpha, HP1A, Heterochromatin Protein 1 Homolog alpha)
Description:
CBX5, also known as Chromobox protein homolog 5, is a regulatory protein belonging to the Polycomb group proteins family with two chromo domains. CBX5 associates with chromosomes during mitosis, specifically with chromatin during metaphase and anaphase. A component of the centromeric and pericentromeric heterochromatin, CBX5 may interact with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. CBX5 recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. This methyl-lysine binding protein localized at heterochromatin sites is also known to mediate gene silencing and is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.03-0.1ug/ml
ELISA: >1:128,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Hela lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human CBX5. Species sequence homology: chinese hamster, canine, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rohr O, Lecestre D, Chasserot-Golaz S, Marban C, Avram D, Aunis D, Leid M, Schaeffer E. Recruitment of Tat to heterochromatin protein HP1 via interaction with CTIP2 inhibits human immunodeficiency virus type 1 replication in microglial cells. J Virol. 2003 May;77(9):5415-27. PMID: 12692243.