Technical Data
C2098-87B
CBX8 (chromobox homolog 8 (Pc class homolog, Drosophila), Chromobox protein homolog 8, hPc3, HPC3, Pc3, PC3, Polycomb 3 Homolog, RC1, Rectachrome 1)
Description:
The family of Heterochromatin Proteins ("Chromobox Homolog", CBX) are highly conserved adapter molecules, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
NIH/3T3 nuclear extract, HeLa cell nuclear extract

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
GST-tagged recombinant protein corresponding to the postchromo domain of CBX8. Cellular Localization: Nucleus
Purity:
Purified by Protein A affinity chromatography with GST depletion.
Form
Supplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes the postchromo domain of CBX8. Species Crossreactivity: mouse, human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.