Technical Data
C2099-67G
CCR3 (CD193, CC Chemokine Receptor Type 3, CC CKR 3, CC CKR3, B Chemokine Receptor, CMKBR3, Eosinophil CC Chemokine Receptor 3, Eosinophil Eotaxin Receptor, MGC102841)
Description:
Human immunodeficiency virus (HIV) and related virus require coreceptors to infect target cells. Some G protein- coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, STRL33, and CX3CR1 in the chemokine receptor family were recently identified as HIV coreceptors. CCR5, CXCR4 and CCR3 are the principal receptors for HIV fusion and entry of target cells (1-4). CCR3 facilitates infection by a subset of virus. CCR3 and CCR5 promote efficient infection of microglia, the major target cells in the CNS (3,4). High levels of CCR3 and
CXCR4 expression were found on the neurons from both the central and peripheral nervous systems (5). The CCR3 ligand, eotaxin, and an anti-CCR3 antibody inhibited HIV infection of microglia. These results indicate CCR3 plays an important role in HIV infection of CNS.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat spleen cell lysate

Control Peptide:
C2099-67G1: CCR3, NT, Control Peptide (Chemokine Receptor 3, CC-CCR-3, CKR3)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, 14aa, near the N- terminus of human CCR3 (Genbank accession No. NP_847899).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human CCR3. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Feng Y, Broder CC, Kennedy PE, et al. Science 1996; 272:872-7. 2. Deng H, Liu R, Ellmeier W, et al. Nature 1996; 381:661-6. 3. Choe H, Farzan M, Sun Y, et al. Cell 1996; 85:1135-48. 4. He J, Chen Y, Farzan M, et al. Nature 1997; 385:645- 9. 5. Zhang L, He T, Talal A, et al. J. Virol. 1998; 72:5035- 45. 6. Combadiere C, Ahuja SK, Murphy PM. J. Biol. Chem. 1995; 270:16491-4.