Technical Data
C2099-67R
CCR3 (CD193, CC Chemokine Receptor Type 3, CC CKR 3, CC CKR3, B Chemokine Receptor, CMKBR3, Eosinophil CC Chemokine Receptor 3, Eosinophil Eotaxin Receptor, MGC102841)
Description:
Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry: 10ug/ml
Optimal dilution determined by the researcher.

Positive control:
Human spleen tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 14aa near the carboxy terminus of human CCR3 (NP_847899).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human CCR3. Species crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Feng, Y., et al., (1996), “HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor”, Science, 272: 872-877 2.Deng, H., et al., (1996), “Identification of a major co-receptor for primary isolates of HIV-1”, Nature, 381: 661-666 3.Choe, H., et al., (1996), “The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates”, Cell, 85: 1135-1148 4.He, J., et al., (1997), “CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia”, Nature, 385: 645-649 5.Zhang, L. et al., (1998), “In vivo distribution of the human immunodeficiency virus/simian immunodeficiency virus coreceptors: CXCR4, CCR3 and CCR5”, J. Virol., 72: 5035-50456.Combadiere, C., et al., (1995), “Cloning and functional expression of a human eosinophil CC chemokine receptor”, J. Biol. Chem., 270: 16491-16494