Technical Data
C2099-67S
CCR3 (CD193, CC Chemokine Receptor Type 3, CC CKR 3, CC CKR3, B Chemokine Receptor, CMKBR3, Eosinophil CC Chemokine Receptor 3, Eosinophil Eotaxin Receptor, MGC102841)
Description:
CCR3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR3 has been reported in blood, brain, lung, bone marrow, kidney, liver, lymph node, placenta, skin, spleen, thymus, thyroid, uterus and vessel. Within these tissues, CCR3 is expressed in specific cell types, including eosinophils, basophils, Th2 cells, and mast cells. The agonists for CXCR3 (IP-10, mig, and I-TAC) act as antagonists for CCR3 by competing for the binding of eotaxin to CCR3. The 356 amino acid CCR3 receptor is expressed on human eosinophils, basophils, neutrophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor cells, Th2-like lymphocytes, and keratinocytes. CCR3 is thought to play a key role in allergic diseases such as bronchial asthma and allergic rhinitis. CCR3 is a co-receptor for HIV-1 and HIV-2 and the binding of eotaxin with CCR3 has been shown to inhibit HIV infection in some cell types. The CCR3 receptor has been reported to be upregulated by high affinity Fc IgE receptor ligation.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:250
Immunocytochemistry: 1:400
Optimal dilutions to be determined by the researcher.

IHC Positive Control:
Paraffin sections of mouse spleen

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
~2mg/ml
Immunogen:
Synthetic peptide corresponding to aa5-29, TDEIKTVVESFETTPYEYEWAPPC at the N-terminal of mouse CCR3. Cellular Localization: Integral membrane protein
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 1mg/ml BSA, sodium azide.
Specificity:
Recognizes mouse CCR3. Peptide sequence is < 50% identical to other mouse chemokine receptors in this region.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gao, J.L., et al., J. Biol. Chem. 270(29): 17,494-17,501 (1995).