Technical Data
C2099-68W1
CNOT4 (CCR4-NOT Transcription Complex Subunit 4, CCR4-associated Factor 4, E3 Ubiquitin-protein Ligase CNOT4, Potential Transcriptional Repressor NOT4Hp, NOT4)
Description:
CNOT4 is a 575aa protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal. Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.05-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human skeletal muscle lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa50-100 of human CNOT4.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human CNOT4. Species Crossreactivity: chicken, bovine, mouse, rat, xenopus. Species sequence homology: pufferfish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Albert, T.K. et al. Nucleic Acids Res. 28:809-817 (2000). 2. Matsuoka, S. et al. Science. 316:1160-1166 (2007). 3. Albert, T.K. et al. EMBO J. 21:355-364 (2002).