Technical Data
CCR10 (C-C Chemokine Receptor 10, CC-CCR-10, GPR2)
Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.

Cellular Localization: Cell Membrane

Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
As reported
Synthetic peptide: VSWGHYSGDEEDAYSAEPLPELC, corresponding to amino acids 9-31 of Human CCR10.
Purified by immunoaffinity chromatography.
10 mM KHPO4, 140 mM NaCl, pH 7.2.
Peptide sequence is < 50% identical to other human chemokine receptors in this region. Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
<b>This product has been used in:</b>Wang W <i>et al.</i> Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2). <i>J Biol Chem</i> <b>275</b>:22313-23 (2000). <PUBMED:10781587>Zaballos A <i>et al.</i> Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. <i>J Immunol</i> <b>162</b>:5671-5 (1999). <PUBMED:10229797>
3. Murakami, T., et al. Immune evasion by murine melanoma mediated through CC chemokine receptor-10. J. Exp. Med. 198(9):1337-1347, 2003.
4. Luttichaux IV, et al. Human adult CD34() progenitor cells functionally express the chemokine receptors CCr1, CCR4, CCR7, CXCR5, and CCR10 but not CXCR4. Stem Cell Dev. 14(3):329-336, 2005.
5. Notohamiprodjo, M., et al. CCR10 is expressed in cutaneous T-cell lymphoma. Int. J. Cancer. 115(4): 641-647, 2005.
6. Hirata, T., et al. Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-assocaited chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. J. Biol. Chem. 279(50): 51775-51782, 2004.