Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.
Cellular Localization: Cell Membrane
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|Synthetic peptide: VSWGHYSGDEEDAYSAEPLPELC, corresponding to amino acids 9-31 of Human CCR10.|
|Purified by immunoaffinity chromatography.|
|10 mM KHPO4, 140 mM NaCl, pH 7.2.|
|Peptide sequence is < 50% identical to other human chemokine receptors in this region. Species Crossreactivity: Crossreacts with Human. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
<b>This product has been used in:</b>Wang W <i>et al.</i> Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2). <i>J Biol Chem</i> <b>275</b>:22313-23 (2000). <PUBMED:10781587>Zaballos A <i>et al.</i> Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. <i>J Immunol</i> <b>162</b>:5671-5 (1999). <PUBMED:10229797>|
3. Murakami, T., et al. Immune evasion by murine melanoma mediated through CC chemokine receptor-10. J. Exp. Med. 198(9):1337-1347, 2003.
4. Luttichaux IV, et al. Human adult CD34() progenitor cells functionally express the chemokine receptors CCr1, CCR4, CCR7, CXCR5, and CCR10 but not CXCR4. Stem Cell Dev. 14(3):329-336, 2005.
5. Notohamiprodjo, M., et al. CCR10 is expressed in cutaneous T-cell lymphoma. Int. J. Cancer. 115(4): 641-647, 2005.
6. Hirata, T., et al. Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-assocaited chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. J. Biol. Chem. 279(50): 51775-51782, 2004.