Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:50,000
Western Blot: 1:500
Immunocytochemistry: 1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 50ug | | 4°C (-20°C Glycerol) | Blue Ice | Human | Goat |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Synthetic peptide TEDYDTTTEFDYGDATPC corresponding to aa 7-24 of the N-terminal extracellular domain of human CCR1. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide, before the addition of glycerol to 40%. |
| Specificity: |
| Recognized human CCR1. Peptide sequence is < 50% identical to other chemokine receptors in this region. No crossreactivity to mouse CCR1. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Molecular Cloning, Functional Expression, and Signaling Characteristics of a C-C Chemokine Receptor: K. Neote, et al., Cell 72, 415 (1993)
2. Preparation of specific polyclonal antibodies to a C-C chemokine receptor , CR1, and determination of CCR1 expression on various types of leukocytes: S. Su,et al.; J.Leukoc.Biol. 60, 658 (1996)
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