Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.
Western Blotting: 1:1,000. Western Blot on membranes from human peripheral blood leukocytes or human T-cell line Jurkatt.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|Synthetic Peptide TEDYDTTTEFDYGDATPC and corresponds to amino acid residues 7-24 of human C-C Chemokine Receptor 1. |
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.09% sodium azide before the addition of 40% glycerol.|
|Peptide sequence shares less than 50% amino acid identity to other Chemokine receptors in this region. Since the peptide sequence shares less than 50% amino acid identity to other chemokine receptors in this region, this antibody only recognizes human receptor. Crossreacts with human.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Neote K, DiGregorio D, Mark J. Y.,Horuk. R.and Schall T.J. 1993. "Molecular Cloning, Functional Expression, and Signaling Characteristics of a C-C Chemokine Receptor." Cell. 72, 415-425. |
2. Su S.B., Mukaida N., Nomura. H and Matsushima. K. 1996. "Preparation of specific polyclonal antibodies to a C-C chemokine receptor, CCR1, and determination of CCR1expression on various types of leukocytes."
3. J. Leukoc., Biol 60(5), 658-666.