Technical Data
C2099-76C
CCR1 (C-C Chemokine Receptor Type 1, C-C CKR-1, CC-CKR-1, CCR-1, Macrophage Inflammatory Protein 1-alpha Receptor, MIP-1alpha-R, RANTES-R, CD191, Cmkbr1)
Description:
CCR1 is a 43kD member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355aa in length. It contains a 34aa N-terminal extracellular domain plus a 50aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1.

Applications:
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2.5ug labels 10e6 cells
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2B12K18Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml after reconstitution
Immunogen:
Recombinant corresponding to aa1-34, aa92-107, aa172-192, aa265-281 from mouse CCR1 expressed in E.coli.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Specificity:
Recognizes mouse CCR1. Does not react with recombinant mouse CCR4, 5, or 8 is observed.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.