Technical Data
C2099-80
CCR2 (Chemokine Receptor 2, CC-CKR-2)
Description:
Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:30,000
Western Blot: 1:500
Immunohistochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide CGEEVTTFFDYDYGAPCHKFDVKQ corresponding to the N-terminal extracellular domain of human CCR2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide before the addition of 40% glycerol.
Specificity:
Recognizes human CCR2. Species sequence homology: < 50% identical to other chemokine receptors in this region.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Charo,I.F., Myers,S.J., Herman,A., Franci,C., Connolly,A.J. and Coughlin,S.R. Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternate splicing of the carboxyl-terminal tails. Proc. Natl. Acad. Sci. U.S.A. 91, 2752-2756 (1994)