Technical Data
C2099-85
CCR3 (CD193, CC Chemokine Receptor Type 3, CC CKR 3, CC CKR3, B Chemokine Receptor, CMKBR3, Eosinophil CC Chemokine Receptor 3, Eosinophil Eotaxin Receptor, MGC102841)
Description:
Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.

Application(s):
ELISA: 1:60,000
Western Blotting: 1:1,000
Immunohistochemistry: 1:300

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
As reported
Immunogen:
CVETFGTTSYYDDVGLLCEKADTRALMA 28 amino acid synthetic. Corresponding to aa 8-34 (with N-terminal cysteine added for coupling) of the N-terminal domain of human CCR3.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide before the addition of 40% glycerol.
Specificity:
Peptide sequence is < 50% identical to other human chemokine receptors in this region. Recognized human CCR3. Crossreacts with human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Auger, G.A., et al. 2002. Eur. J. Immunol. 32, 1052;
2. Humbles, A.A., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 147
3. Power, C.A., et al. 1995. J. Biol. Chem. 270, 19495
4. Human CCR3 is a receptor for CC chemokine eotaxin-1 and oetaxin-2. CCR3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR3 protein contains 356 amino acid. Antibody to human CCR3 receptor was generated to a synthetic peptide derived from the amino terminal extracellular domain of the human CCR3 receptor. Goat anti-CCR3 IgG antibody binds to human eosinophils at a dilution of > 1.500.