Chemokine receptors are specialized proteins that function at the surface of cells, and enable chemokines to trigger internal cellular processes. Chemokines and their receptors play a pivotal role in lymphocyte trafficking, recruiting and recirculation. Chemokines act through seven transmembrane G protein-coupled receptors. The chemokine system is increasingly recognized to play an important role in lymphocyte biology.
Western Blotting: 1:1,000
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|CVETFGTTSYYDDVGLLCEKADTRALMA 28 amino acid synthetic. Corresponding to aa 8-34 (with N-terminal cysteine added for coupling) of the N-terminal domain of human CCR3. |
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide before the addition of 40% glycerol.|
|Peptide sequence is < 50% identical to other human chemokine receptors in this region. Recognized human CCR3. Crossreacts with human.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Auger, G.A., et al. 2002. Eur. J. Immunol. 32, 1052; |
2. Humbles, A.A., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 147
3. Power, C.A., et al. 1995. J. Biol. Chem. 270, 19495
4. Human CCR3 is a receptor for CC chemokine eotaxin-1 and oetaxin-2. CCR3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR3 protein contains 356 amino acid. Antibody to human CCR3 receptor was generated to a synthetic peptide derived from the amino terminal extracellular domain of the human CCR3 receptor. Goat anti-CCR3 IgG antibody binds to human eosinophils at a dilution of > 1.500.