Technical Data
CCR1 (Chemokine receptor 1, CC-CCR-1)
Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Suitable for detection of CCR1 expression by Western blotting. A band of approx. size 42 kD should be detected. Western blot analysis of 10ug of total cell lysate from A357 cells with antibody at 2 g/ml dilution.

Recommended Dilution:
Western blot: 1-3ug/ml

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide of human CCR1 protein.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Cross reacts with mouse CCR1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Murphy, P. M.. 1996. Cytokine Growth Factor Rev. 7: 47. 2. Neote, K., et al., Cell 72: 415. 3. Gao, J. L., et al.,. J. Biol. Chem. 270: 17494 4. Gao, J. L., et al., Biochem. Biophys. Res. Commun. 223: 679 5. Gao, J. L., et al., 1. J. Exp. Med. 185: 1959. 6. Broxmeyer, H. et al., J. Exp. Med. 189: 1987.