Technical Data
C2099-91A
CCR2 (Chemokine receptor 2, CC-CCR-2, CKR2)
Description:
ELISA positive samples that are still undergoing R&D testing. Available testing data will be listed under the Applications section. Often times, multiple samples will be included, differing in the amino acid sequence used to raise the immune sera.

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:100-1:1000

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to amino acids 352-366 of human CCR2.
Purity:
Purified
Form
Supplied as a liquid in glycerol, 0.1% sodium azide.
Specificity:
Recognizes Human CCR2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Murphy, P. M.. 1996. Chemokine receptors: structure, function and role in microbial pathogenesis. Cytokine Growth Factor Rev. 7:47.
2. Neote, K., D. DiGregorio, J. Y. Mak, R. Horuk, T. J. Schall. 1993. Molecular cloning, functional expression, and signaling characteristics of a C-C chemokine receptor. Cell 72:415.
3. Gao, J. L., P. M. Murphy. 1995. Cloning and differential tissue-specific expression of three mouse chemokine receptor-like genes, including the gene for a functional macrophage inflammatory protein-1 receptor. J. Biol. Chem. 270:17494
4. Gao, J. L., A. I. Sen, M. Kitaura, O. Yoshie, M. E. Rothenberg, P. M. Murphy, A. D. Luster. 1996. Identification of a mouse eosinophil receptor for the CC chemokine eotaxin. Biochem. Biophys. Res. Commun. 223:679
5. Gao, J. L., T. A. Wynn, Y. Chang, E. J. Lee, H. E. Broxmeyer, S. Cooper, H. L. Tiffany, H. Westphal, J. Kwon-Chung, P. M. Murphy. 1997. Impaired host defense, hematopoiesis, granulomatous inflammation and type 1-type 2 cytokine balance in mice lacking CC chemokine receptor 1. J. Exp. Med. 185:1959.
6. Broxmeyer, H. E., S. Cooper, G. Hangoc, J. L. Gao, P. M. Murphy. 1999. Dominant myelopoietic effector functions mediated by chemokine receptor CCR1. J. Exp. Med. 189:1987.