Technical Data
C2099-91C
CCR2, NT (Chemokine Receptor 2, CC-CCR-2, CKR2)
Description:
CCR2, C-C chemokine receptor type 2, is a G protein-coupled, seven-transmembrane domain receptor protein. It has been found to be a monocyte chemoattractant protein 1(MCP-1)-specific receptor (1,2). Activation of the CCR2b isoform by MCP-1 binding has been shown to trigger intracelluluar Ca2+ flux and activation of the Jak/Stat pathway (3). A variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors (4), making elucidating their specific functions important for more fully describing the process of HIV infection.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG6k96Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide cooresponding to the N-terminal residues of the human C-C chemokine receptor type 2 .MW: 42-52kD.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Chemokine Receptor 2. Species Crossreactivity: mouse. Does not crossreact with other CKR family members.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Charo, I.F., et al. Proc Natl Acad Sci U S A. 91: 2752–6 (1994). 2. Wong, L.M., et al. . J. Biol. Chem. 272: 1038–45 (1997). 3. Mellado, M., et al. J. Immunol. 161: 805–13 (1998). 4. Doranz, B.J., et al.. Cell 85: 1149–1158 (1996).