Technical Data
C2099-92M
CCR3 (CD193, CC Chemokine Receptor Type 3, CC CKR 3, CC CKR3, B Chemokine Receptor, CMKBR3, Eosinophil CC Chemokine Receptor 3, Eosinophil Eotaxin Receptor, MGC102841)
Description:
CD193, otherwise known as CC chemokine receptor 3 (CCR3), a seven-helix transmembrane G-protein coupled receptor which, along with CCR5 and CXCR4, acts as a principal receptor for the fusion and entry of HIV into target cells. CD193 is expressed by eosinophils, basophils, mast cells, microglia, Th2 helper cells and some epithelial cells.

CD193 acts as a receptor for several CC chemokines including monocyte chemoattractant proteins (MCP) -1, -2, -3 and -4, MIP-1a, MIP-1 beta, and RANTES. The eotaxin subgroup of CC chemokines act as specific agonists for CD193, and this interaction has become a focus area for studies into the infection of microglia by HIV (2) and also allergy research (3).

Applications:
Suitable for use in Immunohistochemistry (paraffin) and Western Blot. Other applications not tested.

Recommended Dilution:
immunohistochemistry (paraffin): 10ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control: human spleen

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to a 14aa sequence located near human CD193 carboxy-terminus.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human CD193. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Combadiere, C. et al. (1995) Cloning and functional expression of a human eosinophil CC chemokine receptor*. J. Biol. Chem. 270: 16491-16494. 2. He, J. et al. (1997) CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 385: 645-649. 3. Holse, M. et al. (2006) CCR3, CCR5, CCR8 and CXCR3 expression in memory T helper cells from allergic rhinitis patients, asymptomatically sensitized and healthy individuals. Clin. Mol. Allergy. 4: 6.