Technical Data
C2099-99G
CCR5 (Chemokine (C-C motif) Receptor 5, C-C Chemokine Receptor Type 5, CCCKR5, CC-CKR-5, C-C CKR-5, CCR-5, AM4-7, CD195, CHEMR13, CKR5, CMKBR5, FLJ78003, HIV-1 Fusion Coreceptor, IDDM22)
Description:
CCR5 (chemokine (C-C motif) receptor 5) also known as CD195 is a receptor for a number of C-C-chemokines, including MIP-1-alpha, MIP-1-beta and RANTES. CD195 is predominantly expressed on T cell, macrophages, dendritic cells and microglia. It is one of the major corecptors for HIV infectivity. CD195 is expressed at low density on activated cells. For the best immunofluorescent staining results, it may be best to maximise signal by using a sensitive three-layer staining technique or a bright fluorochrome for detection.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10B939Affinity Purified
SizeStorageShippingSourceHost
250ug4C/-20C (No Glycerol)Blue IceMouseHamster
Concentration:
~0.5mg/ml
Immunogen:
Mouse CCR5.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes mouse CCR5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.