Technical Data
C2100-16D
CCR1 (Chemokine Receptor 1, CC-CCR-1) (FITC)
Description:
CCR1 (C-C chemokine receptor 1; also known as MIP-1a receptor and CD191) is a 43kD member of the G protein-coupled receptor 1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes, and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent agonists after N-terminal processing. Mouse CCR1 is a seven transmembrane protein that is 355aa in length. It contains a 34aa N-terminal extracellular domain plus a 50aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b11D86Affinity Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Recombinant corresponding to mouse CCR1 expressed in E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS 0.5% BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes mouse CCR1 (FITC).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.