Technical Data
C2100-16E
CCR1 (Chemokine Receptor 1, CC-CCR-1) (PE)
Description:
CCR1 (C-C chemokine receptor 1; also known as MIP-1a receptor and CD191) is a 43kD member of the G protein-coupled receptor 1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes, and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a /CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent agonists after N-terminal processing. Mouse CCR1 is a seven transmembrane protein that is 355aa in length. It contains a 34aa N-terminal extracellular domain plus a 50aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCRI respectively.

Applications:
Suitable for use Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul per 10e6 cells
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cells with B cells from Balb/c mice.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b11D87Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceMouseRat
Concentration:
~0.025mg/ml
Immunogen:
E. coli-derived recombinant mouse CCR1. Extracellular regions (aa1-34, aa92-107, aa172-192, aa 65-281); UniProt Accession Number P51675
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, up to 0.5% BSA , 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes mouse CCR1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.