Technical Data
C2100P
CCR8, Blocking Peptide (TER1, CKR-L1, ChemR1, Chemokine Receptor 8, CC-CKR-8)
50ug
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Technical Data |
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C2100P |
CCR8, Blocking Peptide (TER1, CKR-L1, ChemR1, Chemokine Receptor 8, CC-CKR-8) |
50ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Peptide corresponding to amino acids 183 to 201 of human CCR8, which locate in the second extracellular loop (1-3). CCR8 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human and murine CCR8 were cloned and designated TER1, CKR-L1, and ChemR1 (1-4). The encoded seven transmembrane protein was identified as the receptor for human CC chemokine I-309 and renamed CCR8. Recently, CCR8 was found to serve as a coreceptor for diverse T-cell tropic, dual-tropic and macrophage-tropic HIV-1 strains (5). CCR8 mediates CC chemokine I-309 induced monocyte chemoattraction and HIV-1 envelope fusion and virus infection, which can be prevented by the CCR8 ligand I-309. CCR8 is expressed in spleen, thymus and T lymphoblastic cell lines. Applications: Suitable for use in blocking the activity of anti-CCR8 (Catalog #C2100). Recommended Dilution: Blocking: Incubating the pepetide with equal volume of antibody for 30 minutes at 37°C usuallly completely blocks the antibody activity in Western Blot. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: Synthetic peptide Purity: Purified 60-70% Concentration: 0.2mg/ml Form: Supplied as a liquid in PBS, pH .2, 0.1% BSA, and0.02% sodium azide. Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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1. Napolitano, M., et al., J. Immunol., 1996, 157:2759-63. 2. Zaballos, A., et al., Biochem. Biophys. Res. Commun., 1996, 227:846-53. 3. Samson, M., et al., Eur. J. Immunol., 1996, 26:3021-8. 4. Goya, I., et al., J. Immunol. 1998, 160:1975-81. 5. Horuk, R., et al., J. Biol. Chem., 1998, 273:386-91.
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