Technical Data
C2251-02B
CD1 (Biotin)
Description:
Porcine CD1 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It has a domain organization similar to that of MHC class I molecules and is expressed in association with Beta2-microglobulin. CD1 is found on B cells, macrophages and immature thymocytes. There is evidence for a role of CD1 in presentation of lipids and peptides to T cells.1-3

Applications:
Flow cytometry1
Immunohistochemistry (acetone-fixed, frozen tissue sections)
Immunoprecipitation1-3

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k5K103Purified
SizeStorageShippingSourceHost
500ug-20CBlue IcePorcineMouse
Concentration:
~0.5mg/ml
Immunogen:
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Specificity:
Recognizes porcine CD1, Mr 43-49kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Denham, S., M. Shimizu, A.T. Bianchi, et al. 1994. Vet. Immunol. Immunopathol. 43:259.
2. Saalmuller, A. 1996. Immunol. Today 17:352.
3. Pescovitz, M.D., J.K. Lunney, and D.H. Sachs. 1984. J. Immunol. 133:368.