Technical Data
C2252-04C
CD1d
Description:
CD1d gene product is a 49kD heavy chain associated with beta-2-microglobulin on cortical thymocytes. Beta-2- microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells.

Applications:
Suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and paraffin sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized mice were fused with cells of the NSO mouse myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H649Affinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Beta G2 - CD1d fusion protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human CD1d gene product.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bonish, B., et al., (2000). J. Immunol. 165: 4076-4085. 2. Agea, E. et al. (2005) JEM. 202: 295-308. 3. Spanoudakis, E. et al. (2009) Blood.113: 2498-507. 4. Moll, M. et al. (2010) Blood. 116: 1876-84. 5. Im, J. et al. (2006) J. Immunol. 177: 5226-5235 6. Zeissig, S. et al. (2010) J Clin Invest. 120: 2889-99.