Technical Data
CD3 (Early T Cell Marker)
Human CD3, a glycoprotein, consists of five polypeptide chains (gp20, gp26, p16, p20 & p28). The CD3 complex is involved in T cell activation and signal transduction. The CD3 antigen is T cell specific, and normally present in resting and activated T lymphocytes. It is also present in peripheral blood T cells and thymocytes. CD3 antibody is specific for T cells, and often used to distinguish precursor T cell lymphoblastic lymphoma from its B cell counterpart.

Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen
Immunohistochemistry (Formalin-fixed paraffin-embedded): Pepsin digestion is required for optimal staining.
Optimal dilutions to be determined by the researcher.

Expected Staining Pattern:

Positive Control:
Tonsil or Thymus

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceHumanRabbit
Synthetic peptide derived from human CD3.
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Recognizes both normal and neoplastic human T cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mason DY, et al. J Clin Pathol 42:1194-1200, 1989. 2. Mason DY, et al. J Clin Pathol 41:121-127, 1988. 3. Campana D, et al. J Immunol 138: 648-655, 1987. 4. Chetty R & Gatter K. J Pathol 173:303-307, 1994.