Technical Data
C2254-02
CD3 (Early T Cell Marker)
Description:
Human CD3, a glycoprotein, consists of five polypeptide chains (gp20, gp26, p16, p20 & p28). The CD3 complex is involved in T cell activation and signal transduction. The CD3 antigen is T cell specific, and normally present in resting and activated T lymphocytes. It is also present in peripheral blood T cells and thymocytes. CD3 antibody is specific for T cells, and often used to distinguish precursor T cell lymphoblastic lymphoma from its B cell counterpart.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded tissue sections.
Optimal dilutions to be determined by the researcher.

Epitope Retrieval:
Not Required. HIER exhibited weaker staining than Pepsin pretreatment.

Enzyme Digestion:
Required

Expected Staining Pattern:
Membrane

Positive Control:
Tonsil or Thymus

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1ml-20°CBlue IceHumanRabbit
Concentration:
~0.05mg/ml
Immunogen:
Synthetic peptide derived from human CD3.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes both normal and neoplastic human T cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mason DY, et al. J Clin Pathol 42:1194-1200, 1989. 2. Mason DY, et al. J Clin Pathol 41:121-127, 1988. 3. Campana D, et al. J Immunol 138: 648-655, 1987. 4. Chetty R & Gatter K. J Pathol 173:303-307, 1994.