Technical Data
C2254-56JX
CD3 (Azide Free)
Description:
CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28kD and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and In vitro activation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15K205Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human thymocytes and peripheral blood lymphocytes from a patient with Sezary cell leukemia.
Purity:
Purified
Form
Supplied as a liquid in PBS. No preservative added.
Specificity:
Recognizes the 20kD epsilon chain of the human CD3/T cell antigen receptor complex.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McMichael, A.K., et al., 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. Knapp, W., B. et al., 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford. 3. Schlossman, S., L. et al., 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford. 4. Barclay, A.N., et al., 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD3 Section, Academic Press, New York, p. 137. 5. Beverly, P.C.L., and P.E. Callard. 1981. Eur. J. Immunol. 11:329.