Technical Data
C2254-56N
CD3 (APC)
Description:
CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28kD and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and In vitro activation. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 10ul/10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG15K205Purified
SizeStorageShippingSourceHost
100 Tests4°C Do Not FreezeBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human CD3
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with APC.
Specificity:
Recognizes the 20kD epsilon chain of the human CD3/T cell antigen receptor complex.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McMichael, A.K.,et al eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.2. Knapp, W., et al eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.3. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.4. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD3 Section, Academic Press, New York, p. 1375. Beverly, P.C.L., and P.E. Callard. 1981. Eur. J. Immunol. 11:329.