Technical Data
C2255
CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4)
Description:
CD4, a single chain transmembrane glycoprotein is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lympocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with 60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E+ cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.

CD4-positive T-cells provide helper funciton in pokeweed mitogen driven B-cell differentiation. Moreover, anti-CD4 selectively blocks the autologus mixed lymphocyte reaction (AMLR) with minimal or no effect on the allogeneic MLR (allo-MLR). Blocking of the autoreactivity occurs when either autologus B-lymphocytes or macrophages are used as stimulators. Specificity closely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells.

Applications:
Suitable for use in Flow Cytometry, Radioimmunoprecipitation, and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:4000 (Acetone-fixed frozen sections. Does not stain paraffin embedded tissue sections).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18.F.33Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Normal human blood lymphocytes
Purity:
Purified by column chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.1% sodium azide.
Specificity:
Recognizes and is highly reactive with human CD4 antigen (MW 59kD). Partially blocks binding of monoclonals to Leu-3a. Antibodies do not cross-block with gp129 and do not inhibit HIV induced syncytium formation.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.