Technical Data
C2255-04D
CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4
Description:
Rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. Monoclonal antibodies clustered as CD4 detect most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and macrophages. The CD4 antigen is a 55kD glycoprotein which plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, CD4 acts as a receptor for HIV-1 by binding the viral protein gp120.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100-1:200, Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen and paraffin. Reacts with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
Optimal dilution determined by the researcher

Recommended Secondary Reagents:
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1903-13A: IgG, F(ab’)2, X-Adsorbed (PE) Pab: Gt xMo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo

Recommended Negative Controls:
I1904-78Y: Mouse IgG1 Negative Control

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG16A230Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat Thymocyte Membrane Glycoproteins.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes the rat CD4 cell surface glycoprotein, 55kD. Inhibits proliferation and IL-2 production in the MLR reaction.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Williams, A.F. et al. (1977). Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 12: 663-673. 2. Barclay, A.N. (1981). The localization of populations defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 42: 593-600. 3. Whiteland, J.L. et al. (1995). Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43(3): 313-320. 4. Pelegrí, C. et al. (1995). Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis. J. Rheumatol. 22:124-132. 5. Hofmann, N. et al. (2002). Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats. BMC Immunol. 3:11