Technical Data
C2255-04E
CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (FITC)
Description:
Rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. Monoclonal antibodies clustered as CD4 detect most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and macrophages. The CD4 antigen is a 55kD glycoprotein which plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, CD4 acts as a receptor for HIV-1 by binding the viral protein gp120. CD4 monoclonal antibodies are commonly used in routine testing of blood for CD4+ positive cells and CD4/CD8 ratios in many patient groups, including HIV/AIDS patients. A group of T cell leukemias can be detected using a panel of antibodies including CD4.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: paraffin-embedded material following PLP fixation (periodate-l ysine-paraformaldehyde)
Optimal dilutions to be determined by researcher.

Hybridoma:
Spleen cells from immunized BALB/c mouse were fused with cells of the mouse NS-1 myeloma cell line.

Recommended Negative Controls:
I1904-78X IgG1 Murine Negative Control (For rat tissue) (FITC)

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG16A230Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceRatMouse
Concentration:
~0.5mg/ml
Immunogen:
Rat thymocyte membrane glycoproteins.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).
Specificity:
Recognizes rat CD4 (domain 1) cell surface glycoprotein (55kD) expressed by helper T cells and weakly by monocytes. Inhibits proliferation and IL-2 production in the MLR reaction.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Williams, A.F.et al. (1977). Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 12: 663-673. 2. Barclay, A.N. (1981). The localization of populations defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 42: 593-600. 3. Whiteland, J.L.et al. (1995). Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43(3): 313-320. 4. Pelegrí, C.et al. (1995). Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis. J. Rheumatol. 22:124-132. 5. Hofmann, N. et al. (2002). Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats. BMC Immunol. 3:11.