Technical Data
C2255-31H
CD4 (L3T4) (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (PE/Cy5.5)
Description:
CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested (1,2). CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors (3-6). The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells (1,2). In vivo and in vitro treatment with the effectively depletes CD4+ cells (7,8). The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines (1,8-13).

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, In vivo and in vitro depletion of CD4+ cells, Inhibition of T helper function and Fractionation of CD4+ cells. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.2ug labels 10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin, Cyanine 5.5 (PE/Cy5.5) conjugates will result in a substantial loss of activity. PE/Cy5.5 conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b,k5K206Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Cloned mouse CTL line V41.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, stabilizing agent. Labeled with R-Phycoerythrin, Cyanine 5.5 (PE/Cy5.5).
Specificity:
Mouse CD4/L3T4, Mr 52kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dialynas, D. P., et al. 1983. Immunol. Rev. 74:29. 2. Lakey, E. K., et al. 1986. J. Immunol. 136:3933. 3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473. 4. Wineman, J.P., et al. 1992. Blood 60:1717. 5. Wu, L., R. et al. 1991. Nature 349:71. 6. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1. 7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1. 8. Dialynas, D. P., et al. 1983. J. Immunol. 131:2445. 9. Swain, S. L., et al. 1984. J. Immunol. 132:1118. 10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808. 11. Bluestone, J.A., et al. 1991. Immunol. Rev. 120:5. 12. Moldwin, R. L., et al. 1987. Ann. Inst. Pasteur Immunol. 138:131. 13. Haque, S., et al. 1987. J. Immunol. 139:3207. 14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1.