		Technical Data
C2255-31H	CD4 (L3T4) (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (PE/Cy5.5)

Description:
CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested (1,2). CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors (3-6). The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells (1,2). In vivo and in vitro treatment with the effectively depletes CD4+ cells (7,8). The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines (1,8-13).

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, In vivo and in vitro depletion of CD4+ cells, Inhibition of T helper function and Fractionation of CD4+ cells. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.2ug labels 10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin, Cyanine 5.5 (PE/Cy5.5) conjugates will result in a substantial loss of activity. PE/Cy5.5 conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG2b,k	5K206	Purified

Size		Storage	Shipping	Source	Host
100ug		4°C Do Not Freeze	Blue Ice	Mouse	Rat

Concentration:

~0.1mg/ml

Immunogen:

Cloned mouse CTL line V41.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, stabilizing agent. Labeled with R-Phycoerythrin, Cyanine 5.5 (PE/Cy5.5).

Specificity:

Mouse CD4/L3T4, Mr 52kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Dialynas, D. P., et al. 1983. Immunol. Rev. 74:29. 2. Lakey, E. K., et al. 1986. J. Immunol. 136:3933. 3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473. 4. Wineman, J.P., et al. 1992. Blood 60:1717. 5. Wu, L., R. et al. 1991. Nature 349:71. 6. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1. 7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1. 8. Dialynas, D. P., et al. 1983. J. Immunol. 131:2445. 9. Swain, S. L., et al. 1984. J. Immunol. 132:1118. 10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808. 11. Bluestone, J.A., et al. 1991. Immunol. Rev. 120:5. 12. Moldwin, R. L., et al. 1987. Ann. Inst. Pasteur Immunol. 138:131. 13. Haque, S., et al. 1987. J. Immunol. 139:3207. 14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1.