Technical Data
C2259-15C
CD8, CT (Leu2 T lymphocyte antigen, MAL, OKT8 T Cell antigen, p32, T cell antigen Leu2, T cell surface glycoprotein CD8 alpha)
Description:
The T cell coreceptor CD8 is a cell-surface glycoprotein expressed either as a disulfide-linked
homodimer of two CD8alpha monomers, or a heterodimer of CD8alpha and CD8beta. These receptors interact with ligands, such as major histocompatibility complex (MHC) class I, on the outside of the cell, with proteins inside the cell, such as the tyrosine kinase p56lck, and possibly with proteins on the same cell-surface. CD8 plays an important role in T cell development in the thymus and T cell activation in the periphery.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000. Rat and mouse samples are useful in Western Blot only.
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E193Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues near the C-terminus of human CD8 alpha chain.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human CD8. Species Crossreactivity: mouse and rat (Tested by Western Blot only)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Devine L, et al. Immunol Res, 19(2-3):201-10, 1999. 2. Wong, JS, et al. J Immunol 171:867-874, 2003.