		Technical Data
C2259-33E	CD8 (Leu2 T lymphocyte antigen, MAL, OKT8 T Cell antigen, p32, T cell antigen Leu2, T cell surface glycoprotein CD8 alpha, Suppressor/Cytotoxic T cell) (FITC)

Description:
CD8 is a 32kD transmembrane glycoprotein expressed as either a CD8alpha/alpha homodimer or CD8alpha/beta heterodimer. It is expressed on the “cytotoxic/suppressor” subpopulation of peripheral T cells. CD8 functions primarily as a coreceptor with MHC Class I-restricted TCR’s in antigen recognition.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 10ul/10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	5K61	Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Human CD8.

Purity:

Purified immunoglobulin

Form

Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with FITC.

Specificity:

Recognizes human CD8.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Barclay, A.N., et al., eds. The Leukocyte Antigens Facts Book, 2nd Edition, CD8 Section, Academic Press, New York, p. 149 (1997). 2. Zamoyska, R., Immunity 1: 243 (1994).