Technical Data
C2261-42F
CD10 (Atriopeptidase, CALLA, CD10, Common acute lymphocytic leukemia antigen, DKFZp686O16152, Enkephalinase, EPN, MGC126681, MGC126707, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11, SFE, Skin fibroblast elastase)
Description:
Human CD10 is a well known marker for common acute lymphoblastic leukemia antigen (CALLA). CD10 is expressed on normal early B cells in bone marrow and is also on fetal thymocytes. CD10 expression is lost as the T and B lineage cells mature and only appears again on activated germinal center B cells. CD10 is present on neutrophils. CD10 is a neutral endopeptidase and hydrolyzes peptide bonds on the amino side of hydrophobic amino acids.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 5x10e5 cultured Raji cells were incubated 45 min on ice with 80ul of anti-CD10 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.21 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kSN5c/L4-1A1Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Membrane preparation of human B-lineage leukemia cells
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.
Specificity:
Recognizes human CD10.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. H. Matsuzaki, et al, (1987) Cancer Research 47:2160-2166. 2. W.C. Biddle, et al, (1989) Leukemia Research 13:699-707. 3. H. Hara, et al, (1988) Cancer Research 48:4673-4680. 4. Leukocyte Typing V (S.F. Schlossman,et al, eds.) Oxford University Press, Oxford, (1995) p. 505-507.