		Technical Data
C2261-42F	CD10 (Atriopeptidase, CALLA, CD10, Common acute lymphocytic leukemia antigen, DKFZp686O16152, Enkephalinase, EPN, MGC126681, MGC126707, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11, SFE, Skin fibroblast elastase)

Description:
Human CD10 is a well known marker for common acute lymphoblastic leukemia antigen (CALLA). CD10 is expressed on normal early B cells in bone marrow and is also on fetal thymocytes. CD10 expression is lost as the T and B lineage cells mature and only appears again on activated germinal center B cells. CD10 is present on neutrophils. CD10 is a neutral endopeptidase and hydrolyzes peptide bonds on the amino side of hydrophobic amino acids.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 5x10e5 cultured Raji cells were incubated 45 min on ice with 80ul of anti-CD10 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.21 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	SN5c/L4-1A1	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Membrane preparation of human B-lineage leukemia cells

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.

Specificity:

Recognizes human CD10.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. H. Matsuzaki, et al, (1987) Cancer Research 47:2160-2166. 2. W.C. Biddle, et al, (1989) Leukemia Research 13:699-707. 3. H. Hara, et al, (1988) Cancer Research 48:4673-4680. 4. Leukocyte Typing V (S.F. Schlossman,et al, eds.) Oxford University Press, Oxford, (1995) p. 505-507.