Technical Data
C2262-27
CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL) (FITC)
Description:
CD11a/CD18alpha1 beta2 leukocyte integrin is a member of a family of heterodimeric receptors which mediate cellular adhesion reactions.(1) The CD11a LFA-1 antigen is present on >97% thymocytes, >95% blood lymphocytes, 79% bone marrow cells and certain lymphoma and macrophage-like cell lines.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1ug to stain 1x10e6 cells.
Immunohistochemistry: Acetone fixed frozen.
Optimal dilutions to be determined by the researcher.

Source: Tissue culture

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a1.BB.623Affinity Purified
SizeStorageShippingSourceHost
500ug4C (-20C Glycerol)Blue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
CD11a.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.
Specificity:
Recognizes the alpha subunit of the mouse leukocyte function associated antigen-1 (LFA-1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wuthrich, R. P. et al. (1991) Cell Immunol. 144:22