		Technical Data
C2262-27	CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL) (FITC)

Description:
CD11a/CD18alpha1 beta2 leukocyte integrin is a member of a family of heterodimeric receptors which mediate cellular adhesion reactions.(1) The CD11a LFA-1 antigen is present on >97% thymocytes, >95% blood lymphocytes, 79% bone marrow cells and certain lymphoma and macrophage-like cell lines.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1ug to stain 1x10e6 cells.
Immunohistochemistry: Acetone fixed frozen.
Optimal dilutions to be determined by the researcher.

Source: Tissue culture

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	1.BB.623	Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		4°C (-20°C Glycerol)	Blue Ice	Mouse	Rat

Concentration:

~0.5mg/ml

Immunogen:

CD11a.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.

Specificity:

Recognizes the alpha subunit of the mouse leukocyte function associated antigen-1 (LFA-1).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Wuthrich, R. P. et al. (1991) Cell Immunol. 144:22