Technical Data
C2262-33U
CD11a (Integrin alpha-L, CD11 Antigen-like Family Member A, Leukocyte Adhesion Glycoprotein LFA-1 alpha Chain, LFA-1A, Leukocyte Function-associated Molecule 1 alpha Chain, ITGAL)
Description:
CD11a is a 170-180kD type I transmembrane glycoprotein also known as LFA-1a chain and integrin aL subunit. CD11a non-covalently associates with integrin B2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Blocking of Cell-cell Interaction and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5ug/10e6 cells in 100ul
Immunohistochemistry: acetone-fixed, frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kHI111Affinity Purified
SizeStorageShippingSourceHost
100ug -20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human CD11a
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human CD11a. Species Crossreactivity: chimpanzee, baboon, cynomolgus, rhesus, equine, bovine, sheep, canine, rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Knapp, W.1989. Leucocyte Typing IV. Oxford University Press, New York. 2. Leite, F., et al. 2002. Infec. Immun. 70:4336. 3. Jiang, Y., et al. 2005. Clin. Hemorheol. Microcircul. 32:261. 4. Béchard, D., et al. 2001. J. Immunol. 167:3099. 5. Sithu, S.D., et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. 6. Choi, E.Y., et al. 2008. Blood 111:3607. 7. Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 8. Lub, M., et al. 1995. Immunol. Today 16:479. 9. Parsons, J. 1996. Curr. Opin. Cell Biol. 8:146.