		Technical Data
C2262-34G	CD11b, CD18 (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor)

Description:
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and as an inhibitor of C3bi binding to its receptor. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:50
Immunohistochemistry (Frozen and PLP-fixed, paraffin sections, not formalin-fixed): Requires protein digestion pre-treatment e.g. trypsin or pronase.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	2Q919	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Rat

Concentration:

~0.5mg/ml

Immunogen:

T cell enriched splenocytes from B10 mice.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid, before the addition of glycerol to 40%.

Specificity:

Recognizes the native mouse and human alphaM integrin chain (170kD, CD11b), which exists as a heterodimer with the beta 2 integrin (95kD, CD18). Stains polymorphonuclear cells, monocytes and NK cells. Variable activity is reported with tissue macrophages. Blocks C3bi binding to its receptor.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Springer, T., et al., Eur. J. Immunol. 9: 301-306 (1979). 2. Springer, T., Nature 346: 425-434 (1990). 3. Beller, D.I., et al., J. Exp. Med. 156: 301-306 (1982). 4. Whiteland, J.L., et al., J. Histochem. Cytochem. 43: 313-320 (1995).