Technical Data
C2262-34H
CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor)
Description:
CD11b is also known as CD11b/CD18, Mac-1, alphaMbeta2 integrin, Cr3, MO-1, C3bi Receptor). CD11b is a marcophage-granulocyte specific antigen. Mac-1 macrophage differentiation antigen is associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1). CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and <1% of thymocytes.

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (acetone fixed tissue, paraffin embedded tissue): 1:100
Immunocytochemistry: 1:100 on cell line HL-60.
Immunoprecipitation: 1:100. Recognizes a protein at 95 and 165kD.
Flow Cytometry: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a2Q902Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Leukemic cells of CML.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human CD11b. Reacts with the CR3-receptor of inactivated C3b component of complement (C3bi).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.