Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Flow Cytometry: 10ul per test in 100ul total
Immunohistochemistry: Frozen tissue sections and paraffin embedded tissue sections following periodate-lysine- paraformaldehyde fixation.
Optimal dilutions to be determined by the researcher.
NSO myeloma cell line
Tissue culture supernatant.
Storage and Stability:
Store at 4°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
|100Tests||4°C (-20°C Glycerol)||Blue Ice||Rat||Mouse|
| Not Determined|
|Rat peritoneal macrophages.|
|Puriified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, 10mM sodium azide, 1mg/ml BSA. Labeled with FITC.|
|Recognizes most macrophages (including resident peritoneal and activated macrophages), kupffer cells, but only about 35% of alveolar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95kD. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Robinson A.P.et al. Immunology 57:239–247 (1985) 2. Barclay A.N., Immunology 42:593–600 (1981) 3. Milligan C.E. et al. J Comparative Neurology 314:125- 135 (1991) 4. Whiteland J.L et al. J. Histochem. Cytochem 43:313- 320. (1995).|