		Technical Data
C2262-34U	CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (FITC)

Description:
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Integrin alpha-m/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. CD-11b is identical with cr-3, the receptor for the ic3b fragment of the third complement component. CD-11b probably recognizes the r-g-d peptide in c3b. Integrin alpha-m/beta-2 is also a receptor for fibrinogen, factor x and icam1. it recognizes p1 and p2 peptides of fibrinogen gamma chain.

Applications:
Suitable for use in Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry: Use 10ul/10e6 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG2b	5C29	Highly Purified

Size		Storage	Shipping	Source	Host
500ug		4°C (-20°C Glycerol)	Blue Ice	Mouse	Rat

Concentration:

~1mg/ml

Immunogen:

Purified mouse LN CD4+ T cells.

Purity:

Purified by ion exchange chromatography.

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Labeled with FITC. Reconstitute with 500ul of sterile 40-50% glycerol , dH2O.

Specificity:

Recognizes mouse CD11b and will block signal through the CD11a molecule.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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